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Welcome to the Integrated Chemicals
environment, also known as ICE.

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In this video,

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we will go over how to build a query
in the ICE Curve Surfer tool.

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This tool lets you view and interact

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with concentration-response curves
from in vitro high-throughput screening

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data in ICE that have
been curated by NICEATM.

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These are referred to as "cHTS data".

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To run a curve surfer query,

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you must specify at least one assay
or chemical or combination of both.

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Select chemicals for your query

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in the "Chemical Input" field on the left.
You can choose an ICE Chemical Quick List

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by clicking on the
"Select Chemicals" button.

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For this example, we will choose the
ER In Vitro Agonist chemical Quick List.

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You can also enter Chemical Identifiers
in the User Chemical Identifier box.

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You can do this by typing in one or
more individual chemical Identifiers.

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For this example, we can type in "50-28-2"
the CASRN for the chemical estradiol.

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You can also paste in a list
of chemical identifiers.

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You can use any combination of these

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identifiers: CASRNs, DTXSIDs, SMILEs and InChiKeys.

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An example of these identifiers is

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provided in the information box
located in the chemical input field.

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ICE has cHTS data
for thousands of chemicals.

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Only chemicals with cHTS data return
results for the curves are for query.

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Chemicals without cHTS data in ICE will
simply be omitted from the output;

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no error message or list of chemicals
with missing data will be returned.

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ICE allows you to run a Curve Surfer

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query without specifying chemicals,
but we advised against doing this.

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If you don't add chemicals to your query,

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the query will return results for all
chemicals with data for your selected

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assays, which will result
in a very large output.

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Add in vitro assay data to your query

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by using the "Data Input" field
to the right of the "Chemical Input" field.

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To select cHTS assays,
click the "Select Assays" button.

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A dialog box will open, showing
two tabs: "cHTS" and "Mode of Action."

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Click on the "cHTS" tab to view curated

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in vitro high-throughput screening assays
from ToxCast and Tox21 organized

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into mechanistic target categories
that have been annotated to help clarify

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the biological interpretation
of assay readouts.

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Click on the "Mode of Action" tab to view
curated in vitro high-throughput

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screening assays from ToxCast
and Tox21 organized by mode of action.

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The mode of action categories group assays
to inform on pathways and functions known

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to potentially contribute to toxicological
endpoints of regulatory relevance.

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Use the check boxes to select the assay
annotation terms of interest

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under either tab. Only assays with cHTS

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data in ICE will be shown
as options for selection.

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For this example, under the "cHTS" tab, we

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will choose the assays annotated
to "Endocrine-related Processes."

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When you are finished selecting
assays, click on "Finished."

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The assay annotation terms you have

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selected will be displayed
in the text box.

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ICE will allow you to run a Curve
Surfer query without specifying assays.

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However, such query will return all cHTS

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data for the specified chemicals in the
query, resulting in a very large output.

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Click on "Run" to execute your query.

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Users can navigate back to the search
input page and modify selections.

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The session will remember
the selections made.

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To reset query parameters, select "Reset."

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See the results video for a guide
to view and query results.

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Thank you for watching our video on how
to build a Curve Surfer query in ICE.

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For more detailed information about

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the Curb Surfer tool,
go to "Help" and select "User Guides."