WEBVTT

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Welcome to the Integrated Chemical
Environment, also known as ICE.

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In this video, we will provide a brief
introduction to the ICE Curve Surfer tool.

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Other videos will explain in detail how
to set up a query using

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the ICE Curve Surfer tool and how
to view and save query results.

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The ICE Curve Surfer tool allows you
to view and interact with concentration-

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response curves from the in vitro high-throughput screening data in ICE

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that have been curated by NICEATM.

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This tool allows users to input one or
more chemicals or assays to view a set

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of concentration response curves
that can be sorted and filtered.

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Both graphics and data can be exported
to save or to build additional

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queries in ICE or other resources.

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Graphs returned after a user query are
single chemical-assay concentration

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response curves accompanied by information
that can help contextualize the curve.

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The AC50 informs on the chemical's
potency in the assay, while the top of

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curve informs on the chemical's efficacy.

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Mechanistic target annotations
for the assay are also provided

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to facilitate biological interpretation.

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Several options are provided to organize
Curve Surfer query results.

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Large queries may span multiple
pages and can be sorted or filtered.

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You can also overlay several concentration
response curves of interest

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to compare them more easily.

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Located throughout the tool are green
information buttons to help you better

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understand key features and results.

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When you hover over a button,
brief explanatory text will appear.

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When you click a button, more
details will appear in a text box that can

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be relocated on the screen
for a clearer view.

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If you encounter a problem using a tool,
click the "Report an Issue" button

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on the left side of the display
to generate an email to ICE support.

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Thank you for watching our introduction
video for the Curve Surfer tool in ICE.

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For more detailed information on the tool,
go to "Help" and select "User Guides".